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Appraise preclinical test compounds and biologics for their capabilities to modulate the immune system.
Flow cytometry may be used to evaluate the efficiency of differentiation protocols While using the STEMdiff™ system, by checking out specific marker expression at Every phase of the differentiation course of action. It can even be useful for other programs, together with cell sorting, immunophenotyping, and purity evaluation.
If cells will not dissociate very well with trituration, go with a longer ACCUTASE™ incubation, up to 12 minutes. If monolayers don't dissociate right after 12 minutes of ACCUTASE™ incubation and trituration, the differentiation was most likely unsuccessful.
The STEMdiff™ system provides a standardized method for differentiating hPSCs into epithelial cells which might be later cultured in 2D or 3D formats based on the investigation desires.
Alternatively, dissociate organoids utilizing Mild Cell Dissociation Reagent (GCDR) without having prior retrieval of organoids from the Matrigel® dome. This may be surely quicker but bears the chance of inadequate cell dissociation due to concerns with Matrigel® and will be extra demanding into the cells.
Going back again into the plate, rinse Every properly with one mL of FACS buffer and transfer the quantity into the 15 mL tube. Be aware: Hold cell suspension on ice immediately after transfer into the tube until able to run FACS.
Here's a list of printed literature that cites culturing of nasal epithelial cells utilizing PneumaCult™ Stem cell society media:
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When introducing GCDR straight to the cultures, pipette up and down multiple occasions to mechanically break up the dome entirely making sure that Stem cell GCDR can act on cells for dissociation.
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